5I2U
Crystal structure of a novel Halo-Tolerant Cellulase from Soil Metagenome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-05-21 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.542 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.766, 88.128, 146.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.770 - 2.200 |
R-factor | 0.21146 |
Rwork | 0.209 |
R-free | 0.25706 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pzt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.295 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.770 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.078 | 0.457 |
Number of reflections | 30246 | |
<I/σ(I)> | 15.9 | 2.7 |
Completeness [%] | 98.3 | 88 |
Redundancy | 6 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.1M TRIS, 25% PEG3350 |