Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-01-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.3861 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 120.510, 120.510, 183.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.942 - 3.000 |
R-factor | 0.1841 |
Rwork | 0.181 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2CMZ |
RMSD bond length | 0.006 |
RMSD bond angle | 0.907 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 3.200 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.110 | 0.500 |
Number of reflections | 16104 | |
<I/σ(I)> | 6.6 | 1.7 |
Completeness [%] | 97.5 | 94.6 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 0.1M TRIS PH 8.7, NANO3 0.4M, PEG 4000 24% |