5I28
Azurin T30R1, crystal form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8943 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 242.478, 105.560, 100.051 |
Unit cell angles | 90.00, 114.42, 90.00 |
Refinement procedure
Resolution | 18.221 - 1.950 |
R-factor | 0.2124 |
Rwork | 0.212 |
R-free | 0.27130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e67 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.194 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.220 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.163 | 2.609 |
Number of reflections | 166299 | |
<I/σ(I)> | 9 | |
Completeness [%] | 99.6 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | sodium malonate |