5I27

Crystal structure of non-myristoylated MMTV matrix protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.918400
Spacegroup name	P 31 2 1
Unit cell lengths	60.875, 60.875, 91.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.670 - 2.050
R-factor	0.2464
Rwork	0.244
R-free	0.29060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.531
Data scaling software	XSCALE
Phasing software	MOLREP (11.0.02)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.730	45.730	2.170
High resolution limit [Å]	2.050	6.100	2.050
Rmerge	0.071	0.029	1.071
Number of reflections	12780
<I/σ(I)>	17.06	44.29	2.01
Completeness [%]	99.7	97.6	99.1
Redundancy	7.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.2M zinc acetate, 20%(w/v) PEG 33500