5I1U
Crystal structure of germacradien-4-ol synthase from Streptomyces citricolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2014-12-06 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.000064 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.369, 74.127, 82.489 |
| Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
| Resolution | 55.125 - 1.500 |
| R-factor | 0.1577 |
| Rwork | 0.157 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pentalenene synthase |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.125 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 98277 |
| <I/σ(I)> | 11.6 |
| Completeness [%] | 99.4 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.1 M Tris, 2.0 M ammonium sulfate, pH 8.5 |
