5I1H

CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV3-53/IGKV3-20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2009-01-27
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	89.380, 89.380, 211.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.222
R-factor	0.23004
Rwork	0.229
R-free	0.26588
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i1i 5i1c
RMSD bond length	0.005
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.222	2.222
Rmerge	0.062	0.406
Number of reflections	24323
<I/σ(I)>	36.2	1.6
Completeness [%]	95.3	52
Redundancy	15.7	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	19% PEG 4K, 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE PH 4.5