5I1E

CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV3-53/IGKV1-39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2009-09-14
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	89.380, 89.380, 212.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.19455
Rwork	0.191
R-free	0.26362
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.213
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC (5.5.0109)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.095	0.451
Number of reflections	14402
<I/σ(I)>	38.3	8.1
Completeness [%]	99.6	96.8
Redundancy	20.6	19.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16% PEG 3K, 0.2 M AMMONIUM SULFATE, 5% DIOXANE