5I16

CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV1-69/IGKV3-11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	212.010, 55.050, 80.290
Unit cell angles	90.00, 97.88, 90.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.19279
Rwork	0.192
R-free	0.22156
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i18 5i19
RMSD bond length	0.005
RMSD bond angle	1.106
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.044	0.245
Number of reflections	71932
<I/σ(I)>	17.8	4.7
Completeness [%]	99.0	97.3
Redundancy	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES PH 6.5, 25% PEG 3350, 0.2 M SODIUM FORMATE