5I14

Truncated and mutated T4 lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	60.088, 61.206, 69.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.021 - 1.745
R-factor	0.2142
Rwork	0.213
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4GBR (N terminal T4L only)
RMSD bond length	0.006
RMSD bond angle	0.942
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.021	1.808
High resolution limit [Å]	1.745	1.745
Rmerge	0.049	1.322
Number of reflections	26668
<I/σ(I)>	22.46	1.33
Completeness [%]	99.5
Redundancy	7.1
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% PEG 6000, 100mM MES pH 5