5I0V
IRON AND COPPER-BOUND P19 FROM CAMPYLOBACTER JEJUNI UNDER OXIDIZING CONDITIONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.220, 73.720, 74.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 1.650 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lzo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.272 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.6.0117) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.070 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.082 | |
| Number of reflections | 36846 | |
| <I/σ(I)> | 15 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.3 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 25-45% PEG 350, 0.1M CHES PH 9 |
