5I0T
Thiosulfate bound Cysteine Dioxygenase at pH 6.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-03-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.600, 57.600, 122.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.600 - 1.370 |
| R-factor | 0.1723 |
| Rwork | 0.169 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ieo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.092 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2386: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.600 | 1.390 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.183 | |
| Number of reflections | 44485 | |
| <I/σ(I)> | 15.9 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 27.3 | 21.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.1 M tri-sodium citrate pH 5.6, 24% PEG 4000, 0.15 M ammonium acetate |
