5I09
tRNA guanine transglycosylase (TGT) in soaked complex with Furanoside-Based lin-Benzoguanine 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-17 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.895 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.989, 65.091, 70.776 |
Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
Resolution | 23.449 - 1.440 |
R-factor | 0.1346 |
Rwork | 0.133 |
R-free | 0.16630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p0d |
RMSD bond length | 0.005 |
RMSD bond angle | 0.787 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1-2155_1492) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.220 | 1.530 |
High resolution limit [Å] | 1.440 | 1.440 |
Rmerge | 0.495 | |
Number of reflections | 74015 | |
<I/σ(I)> | 14.9 | 2.9 |
Completeness [%] | 99.5 | 99.1 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 13% PEG 8000,100mM MES, 1mM DTT, 10% DMSO |