5HZO
GFP mutant S205G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.031, 96.284, 151.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.490 |
| R-factor | 0.17993 |
| Rwork | 0.175 |
| R-free | 0.27598 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1emb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.853 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.540 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.100 | 0.430 |
| Number of reflections | 13474 | |
| <I/σ(I)> | 13.8 | 1.3 |
| Completeness [%] | 96.9 | 12.4 |
| Redundancy | 2.6 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 295 | 1.0 M malate, pH 8.0, Tris, 0.3 M NaCl, 1% n-nonyl-beta-D maltoside |
