5HZO
GFP mutant S205G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-02-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.031, 96.284, 151.591 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.490 |
R-factor | 0.17993 |
Rwork | 0.175 |
R-free | 0.27598 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1emb |
RMSD bond length | 0.014 |
RMSD bond angle | 1.853 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.000 | 2.540 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.100 | 0.430 |
Number of reflections | 13474 | |
<I/σ(I)> | 13.8 | 1.3 |
Completeness [%] | 96.9 | 12.4 |
Redundancy | 2.6 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 295 | 1.0 M malate, pH 8.0, Tris, 0.3 M NaCl, 1% n-nonyl-beta-D maltoside |