5HZM
Human HMT1 hnRNP methyltransferase-like protein 6 (S. cerevisiae)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.25496 |
Spacegroup name | I 41 |
Unit cell lengths | 94.190, 94.190, 109.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.020 |
R-factor | 0.1762 |
Rwork | 0.175 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hc4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.448 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.050 |
High resolution limit [Å] | 2.020 | 5.480 | 2.020 |
Rmerge | 0.065 | 0.028 | 0.986 |
Rmeas | 0.068 | ||
Rpim | 0.026 | ||
Total number of observations | 232791 | ||
Number of reflections | 31311 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 7.4 | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 10% PEG 8000, 0.1 M Tris-HCL pH8.5 |