5HZL
Secreted Internalin-like protein Lmo2445 from Listeria monocytogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.350, 120.350, 62.286 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.840 - 2.750 |
R-factor | 0.19323 |
Rwork | 0.192 |
R-free | 0.22571 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.693 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.137 | 0.761 |
Number of reflections | 26135 | |
<I/σ(I)> | 13.8 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | Protein: 17.5 mg/ml, 0.5 M NaCl, 10 mM Tris pH 8.3, 1 mM TCEP Crystallization condition: PACT B11 (qiagen), 0.2 M Calcium chloride, 0.1 M MES pH 6, 20% (w/v) PEG 6000 |