5HZG
The crystal structure of the strigolactone-induced AtD14-D3-ASK1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.397, 172.979, 186.845 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.070 - 3.300 |
R-factor | 0.24969 |
Rwork | 0.246 |
R-free | 0.31585 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.781 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 49674 | |
<I/σ(I)> | 12.6 | |
Completeness [%] | 99.9 | |
Redundancy | 19.82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291 | 16% PEG 3350, 16mM BICINE pH 9.0, 20mM HEPES pH 7.5, 4.4% w/v Polyacrylic Acid 5100 Sodium salt, 10mM Praseodymium (III) acetate hydrate, 100 mM NDSB-256 |