5HZG
The crystal structure of the strigolactone-induced AtD14-D3-ASK1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.397, 172.979, 186.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.070 - 3.300 |
| R-factor | 0.24969 |
| Rwork | 0.246 |
| R-free | 0.31585 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.781 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 49674 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 19.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 16% PEG 3350, 16mM BICINE pH 9.0, 20mM HEPES pH 7.5, 4.4% w/v Polyacrylic Acid 5100 Sodium salt, 10mM Praseodymium (III) acetate hydrate, 100 mM NDSB-256 |
