5HZB
Crystal structure of GII.10 P domain in complex with 2-fucosyllactose (2'FL)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.680, 78.900, 71.070 |
Unit cell angles | 90.00, 102.29, 90.00 |
Refinement procedure
Resolution | 42.808 - 1.553 |
R-factor | 0.1652 |
Rwork | 0.164 |
R-free | 0.19530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3onu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.089 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.810 | 44.052 | 1.650 |
High resolution limit [Å] | 1.550 | 4.640 | 1.550 |
Rmerge | 0.034 | 0.015 | 0.282 |
Number of reflections | 94121 | ||
<I/σ(I)> | 15.47 | 46.67 | 2.59 |
Completeness [%] | 92.1 | 95.6 | 93.9 |
Redundancy | 2.2 | ||
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Sodium Nitrate, Bis-Tris Propane, PEG 3350 |