5HZ7

High-resolution crystal structure of the minor DNA-binding pilin ComP from Neisseria meningitidis in fusion with MBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	63.570, 68.440, 109.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.048 - 1.430
R-factor	0.1575
Rwork	0.157
R-free	0.19090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hsj
RMSD bond length	0.004
RMSD bond angle	0.744
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.050
High resolution limit [Å]	1.430
Rmerge	0.039	1.096
Number of reflections	88446
<I/σ(I)>	15
Completeness [%]	99.5
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M sodium cacodylate, 25% PEG 4000