5HYZ

Crystal Structure of SCL7 in Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	89.188, 179.054, 59.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.181 - 1.822
R-factor	0.1823
Rwork	0.181
R-free	0.21070
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.729
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.850
High resolution limit [Å]	1.820	4.940	1.820
Rmerge	0.082	0.067	0.394
Rmeas	0.087
Rpim	0.027
Total number of observations	465093
Number of reflections	43031
<I/σ(I)>	15.9
Completeness [%]	99.8	97.3	100
Redundancy	10.8	9.8	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	10% PEG 4000, 10% isopropanol, 0.1M HEPES