5HYX

Plant peptide hormone receptor RGFR1 in complex with RGF1

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-21
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	182.335, 182.335, 87.441
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.022 - 2.596
R-factor	0.1928
Rwork	0.191
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.421
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.596
Number of reflections	33085
Completeness [%]	98.8
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES monohydrate pH 6.0, 22% (v/v) polyethylene glycol (PEG) 400