5HYW
The crystal structure of the D3-ASK1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 |
| Unit cell lengths | 79.268, 79.268, 327.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.890 - 3.010 |
| R-factor | 0.23921 |
| Rwork | 0.235 |
| R-free | 0.31193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hzg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.671 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.890 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 38298 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 94.1 | |
| Redundancy | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 291 | 0.2M Lithium sulfate monohydrate, 0.1M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3350 |
