5HYD
Crystal structure of calcium-free human S100Z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.326, 96.948, 115.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.799 - 2.300 |
| R-factor | 0.2135 |
| Rwork | 0.210 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wce |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.642 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((dev_2621: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.800 |
| Number of reflections | 17889 | |
| <I/σ(I)> | 3.3 | 1 |
| Completeness [%] | 97.1 | 90.9 |
| Redundancy | 5.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M sodium cacodylate, 0.2 M sodium acetate, 30% PEG 8000 |
