5HYC
Structure based function annotation of a hypothetical protein MGG_01005 related to the development of rice blast fungus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 105.097, 105.097, 166.541 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.626 - 2.400 |
R-factor | 0.2087 |
Rwork | 0.206 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hxl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.194 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.630 |
High resolution limit [Å] | 2.400 |
Number of reflections | 18442 |
<I/σ(I)> | 28 |
Completeness [%] | 99.0 |
Redundancy | 25.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 287 | 2.0 M ammonium sulfate, 0.1M Bis-Tris(pH 5.2) |