5HYC

Structure based function annotation of a hypothetical protein MGG_01005 related to the development of rice blast fungus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	I 41 2 2
Unit cell lengths	105.097, 105.097, 166.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.626 - 2.400
R-factor	0.2087
Rwork	0.206
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hxl
RMSD bond length	0.010
RMSD bond angle	1.194
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.630
High resolution limit [Å]	2.400
Number of reflections	18442
<I/σ(I)>	28
Completeness [%]	99.0
Redundancy	25.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	287	2.0 M ammonium sulfate, 0.1M Bis-Tris(pH 5.2)