5HY3
Crystal structure of Escherichia coli toxin LsoA in complex with T4 phage antitoxin Dmd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.431, 112.431, 95.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.750 - 3.100 |
| R-factor | 0.2615 |
| Rwork | 0.259 |
| R-free | 0.30170 |
| Structure solution method | SAD |
| Starting model (for MR) | the Se-Met derivative of this complex |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-2000 (7.04) |
| Data scaling software | HKL-2000 (7.04) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.557 | |
| Number of reflections | 11548 | |
| <I/σ(I)> | 61.93 | 8.39 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 15.2 | 16.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.65M ammonium citrate tribase |
