5HXV
The crystal structure of thermostable xylanase mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-23 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.996, 179.877, 93.338 |
Unit cell angles | 90.00, 103.85, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.17842 |
Rwork | 0.176 |
R-free | 0.22855 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.871 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 153393 |
<I/σ(I)> | 8.6 |
Completeness [%] | 94.2 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG8000, Sodium citrate, Magnesium chloride |