5HXO
Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase with D71M, E75A and H103Y Mutants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2014-12-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 51.683, 51.683, 173.561 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.780 - 2.050 |
R-factor | 0.19338 |
Rwork | 0.191 |
R-free | 0.23646 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jp3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.214 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.043 | 0.412 |
Number of reflections | 15682 | |
<I/σ(I)> | 42.8 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.6 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 291 | 5% (w/v) PEG 8000, 40% MPD, 0.1 M Cacodylate, pH 7.0 |