5HXN
Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M and E75A mutants) from the Wild Tomato Solanum habrochaites
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2013-01-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 51.891, 51.891, 173.286 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.220 - 2.050 |
R-factor | 0.19413 |
Rwork | 0.192 |
R-free | 0.24135 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jp3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.159 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.044 | 0.495 |
Number of reflections | 15386 | |
<I/σ(I)> | 52.3 | 4.3 |
Completeness [%] | 97.4 | 94.6 |
Redundancy | 11 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 291 | 70% 2-Methyl-2,4-pentanediol (MPD), 0.1 M Tris, pH 8.0. |