5HXL
Structure based function annotation of a hypothetical protein MGG_01005 related to the development of rice blast fungus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 72.640, 72.640, 46.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.500 - 1.970 |
R-factor | 0.1755 |
Rwork | 0.172 |
R-free | 0.20740 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.019 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.500 |
High resolution limit [Å] | 1.970 |
Number of reflections | 10166 |
<I/σ(I)> | 17.3 |
Completeness [%] | 98.5 |
Redundancy | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1.8M ammonium citrate |