5HXI
2-Methyl-3-hydroxypyridine-5-carboxylic acid oxygenase, 5HN bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-25 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 49.338, 131.140, 132.282 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.15207 |
| Rwork | 0.151 |
| R-free | 0.17402 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.191 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.130 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.063 | 0.379 |
| Number of reflections | 68494 | |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 8% PEG8000, 0.1M Tris-HCl pH 8.5 |
