5HX7
Metal ABC transporter from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.603, 66.652, 67.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.400 - 1.750 |
| R-factor | 0.1726 |
| Rwork | 0.171 |
| R-free | 0.20130 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.447 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
| Rmerge | 0.121 | 0.070 | 0.859 |
| Rmeas | 0.135 | 0.079 | 0.977 |
| Rpim | 0.059 | 0.035 | 0.454 |
| Total number of observations | 160627 | ||
| Number of reflections | 30563 | ||
| <I/σ(I)> | 8.8 | 2.18 | |
| Completeness [%] | 99.6 | 98.2 | 99.1 |
| Redundancy | 5.3 | 4.8 | 4.4 |
| CC(1/2) | 0.994 | 0.631 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M magnesium chloride, 0.1 M Tris buffer, 20% PEG 8000 |
