5HX0

Crystal structure of unknown function protein Dfer_1899 fromDyadobacter fermentans DSM 18053

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-08
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9792915
Spacegroup name	P 21 21 21
Unit cell lengths	54.698, 85.441, 158.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.847 - 1.851
R-factor	0.1564
Rwork	0.154
R-free	0.19440
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.981
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.073	0.038	0.899
Rmeas	0.078	0.041	0.966
Rpim	0.026	0.014	0.344
Total number of observations	566421
Number of reflections	64380
<I/σ(I)>	10.8
Completeness [%]	100.0	99.5	99.7
Redundancy	8.8	8.1	7.5
CC(1/2)		0.997	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	0.17 M Ammonium sulfate, 0.085M Sodium Acetate, 25.5 % PEG4000, 15 % Glycerol