5HWK
Crystal structure of gama glutamyl cyclotransferease specific to glutathione from yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.779, 62.019, 61.695 |
| Unit cell angles | 113.71, 89.89, 101.80 |
Refinement procedure
| Resolution | 30.143 - 1.344 |
| R-factor | 0.1624 |
| Rwork | 0.161 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hwi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.183 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.390 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.080 | 0.840 |
| Number of reflections | 103046 | |
| <I/σ(I)> | 23.6 | 2 |
| Completeness [%] | 84.5 | 32.3 |
| Redundancy | 10.2 | 6 |
| CC(1/2) | 0.960 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Ammonium Phosphate, 20% PEG3350, 15% Glycerol |
