5HW9
Filamentous Assembly of Green Fluorescent Protein Supported by a C-terminal fusion of 18-residues, viewed in space group P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.4760 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.730, 51.800, 54.790 |
Unit cell angles | 90.00, 95.96, 90.00 |
Refinement procedure
Resolution | 45.480 - 3.000 |
R-factor | 0.1911 |
Rwork | 0.186 |
R-free | 0.23220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hbd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.6) |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.480 | 3.080 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.189 | 0.750 |
Number of reflections | 4965 | |
<I/σ(I)> | 5.81 | 1.24 |
Completeness [%] | 95.1 | 84.4 |
Redundancy | 3.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% 1,6-hexanediol, sodium cacodylate pH 6.5, 5 mM magnesium chloride, 200 mM KCl |