5HW7
Candida albicans FKBP12 apo protein in P21212 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-01 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 35.813, 80.714, 94.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.905 - 2.291 |
R-factor | 0.1839 |
Rwork | 0.179 |
R-free | 0.22760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.423 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.291 | 6.240 | 2.291 |
Rmerge | 0.064 | 0.025 | 0.559 |
Number of reflections | 12808 | ||
<I/σ(I)> | 16.6 | ||
Completeness [%] | 98.8 | 94.3 | 92.3 |
Redundancy | 3 | 2.9 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2M Ammonium Sulfate , 0.1M Citrate pH5.5 |