5HW4
Crystal structure of Escherichia coli 16S rRNA methyltransferase RsmI in complex with AdoMet
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.923, 155.270, 54.604 |
Unit cell angles | 90.00, 93.89, 90.00 |
Refinement procedure
Resolution | 34.943 - 2.211 |
R-factor | 0.204 |
Rwork | 0.203 |
R-free | 0.22950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kwp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.107 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.063 | 0.535 |
Number of reflections | 46162 | |
<I/σ(I)> | 31.5 | |
Completeness [%] | 93.1 | 97.7 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M DL-Malic acid, 20% PEG 3350 |