5HVD

Full length Open-form Sodium Channel NavMs I218C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97718
Spacegroup name	I 4 2 2
Unit cell lengths	109.580, 109.580, 209.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.920 - 2.600
R-factor	0.1936
Rwork	0.193
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS (Oct 15, 2015)
Data scaling software	XDS (Oct 15, 2015)
Phasing software	PHASER (2.5.7)
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Outer shell
Low resolution limit [Å]	2.720
High resolution limit [Å]	2.600
Rmerge	3.600
<I/σ(I)>	1.4
Completeness [%]	99.7
Redundancy	45.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.05 M NaCl, 0.1 M MES buffer, pH 6.5, 37.8% v/v polyethylene glycol (PEG) 400 and 4% ethylene glycol