5HV3

Rifampin phosphotransferase G527Y mutant in complex with AMPPNP from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	77
Detector technology	IMAGE PLATE
Collection date	2015-05-16
Detector	MAR555 FLAT PANEL
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	58.686, 127.990, 140.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.508 - 3.118
R-factor	0.2071
Rwork	0.202
R-free	0.25380
RMSD bond length	0.012
RMSD bond angle	1.668
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.100
Number of reflections	18949
<I/σ(I)>	13.3
Completeness [%]	97.2
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	20%(w/v) PEG3350, 200 mM sodium formate