5HV1
Rifampin phosphotransferase in complex with AMPPNP and rifampin from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-03-19 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 151.090, 151.090, 191.648 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.019 - 3.103 |
| R-factor | 0.253 |
| Rwork | 0.250 |
| R-free | 0.28630 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.129 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.019 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 23960 |
| <I/σ(I)> | 28.8 |
| Completeness [%] | 99.7 |
| Redundancy | 26.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.2 | 277 | 18%(w/v) PEG3350, 100 mM Bicine |
