5HUX
Putative sugar kinases from Synechococcus elongatus PCC7942 in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.104, 46.662, 89.394 |
| Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
| Resolution | 38.345 - 1.960 |
| R-factor | 0.1772 |
| Rwork | 0.174 |
| R-free | 0.22460 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.956 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.075 | 0.233 |
| Number of reflections | 29851 | |
| <I/σ(I)> | 18.8 | 5.75 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 5.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 281 | 0.1M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5000 |
