5HUS
Structure of Candida albicans trehalose synthase regulatory protein C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-06 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 106.940, 106.940, 41.886 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.973 - 1.751 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.21680 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.794 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
| Rmerge | 0.073 | 0.059 | 0.360 |
| Rmeas | 0.081 | 0.066 | 0.420 |
| Rpim | 0.036 | 0.029 | 0.212 |
| Total number of observations | 149128 | ||
| Number of reflections | 27807 | ||
| <I/σ(I)> | 8.7 | ||
| Completeness [%] | 99.9 | 98.6 | 100 |
| Redundancy | 5.4 | 5.2 | 3.6 |
| CC(1/2) | 0.995 | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M sodium citrate, 20% PEG 4000, 10% 2-propanol |
