5HUN
The crystal structure of neuraminidase from A/gyrfalcon/Washington/41088-6/2014 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-26 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 |
Unit cell lengths | 90.652, 90.652, 110.809 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.20262 |
Rwork | 0.201 |
R-free | 0.23704 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.576 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 19878 |
<I/σ(I)> | 47.8 |
Completeness [%] | 99.8 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 0.16 M Calcium Acetate, 0.08 M Sodium Cacodylate:HCl pH 6.5, 14.4% (w/v) PEG 8000, 20% (v/v) Glycerol |