5HUM
The crystal structure of neuraminidase from A/Sichuan/26221/2014 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-25 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 117.335, 76.277, 117.211 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 34.803 - 1.600 |
R-factor | 0.1988 |
Rwork | 0.198 |
R-free | 0.22000 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.681 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.600 |
Number of reflections | 272309 |
<I/σ(I)> | 22 |
Completeness [%] | 99.9 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 0.2M Sodium Acetate, 0.1M Tris:HCL pH 8.5, 16% (w/v) PEG 4000 |