5HUL
Crystal Structure of NadC Deletion Mutant in Cubic Space Group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 179.630, 179.630, 179.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.200 - 2.855 |
| R-factor | 0.17169 |
| Rwork | 0.170 |
| R-free | 0.20518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kwv |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.712 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.057 | 0.815 |
| Number of reflections | 44917 | |
| <I/σ(I)> | 24.1 | 2 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 1 M Ammonium Phosphate, 0.1 M Sodium citrate tribasic/ Citric acid, 0.2 M Sodium Chloride |
