5HU6
Structure of the T. brucei haptoglobin-haemoglobin receptor bound to human haptolgobin-haemoglobin
Replaces: 4X0IExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.975 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 222.290, 56.560, 66.100 |
Unit cell angles | 90.00, 92.97, 90.00 |
Refinement procedure
Resolution | 58.070 - 2.900 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.22640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xol |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.102 | 0.420 |
Number of reflections | 18486 | |
<I/σ(I)> | 6 | 1.9 |
Completeness [%] | 99.1 | 98.7 |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12.5% v/v MPD, 0.03 M NaBr, 0.03M NaI, 0.03M NaF, 0.1 M MES/imidazole pH 6.5, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350 |