5HTS
Crystal structure of shaft pilin spaA from Lactobacillus rhamnosus GG - D295N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95371 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.328, 75.161, 176.476 |
| Unit cell angles | 90.00, 100.30, 90.00 |
Refinement procedure
| Resolution | 37.900 - 2.600 |
| R-factor | 0.24316 |
| Rwork | 0.242 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f44 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.480 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.900 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.054 | 0.274 |
| Number of reflections | 64379 | |
| <I/σ(I)> | 14.4 | 3.6 |
| Completeness [%] | 98.6 | 96.9 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M Tris, 15%(w/v) PEG 20000 |
