5HTL
Structure of MshE with cdg
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-08 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.852, 61.862, 75.621 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.426 - 1.371 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.22110 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.266 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.370 |
| Number of reflections | 234156 |
| <I/σ(I)> | 18.2 |
| Completeness [%] | 95.4 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | Tris 100mM pH 8.0, PEG 3350 20% |
