5HTF
Crystal Structure of PrsA1 from Listeria monocytogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-06-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97941 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.310, 84.030, 114.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.782 - 2.100 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.23420 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.934 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.782 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.064 | 0.984 |
Number of reflections | 37408 | |
<I/σ(I)> | 17.4 | 1.9 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 5.7 | 5.8 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 278 | 26 mg/mL PrsA1 with a 1:1 mixture of 30% MPD (2-methyl-2,4-pentadiol) 100 mM Na Acetate pH 4.5. |