5HT7

Crystal structure of a transition-metal-ion-binding betagamma-crystallin from Methanosaeta thermophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-11-23
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	94.732, 52.821, 51.718
Unit cell angles	90.00, 100.48, 90.00

Refinement procedure

Resolution	24.250 - 1.862
R-factor	0.1842
Rwork	0.182
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.961
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.930
High resolution limit [Å]	1.860	4.000	1.860
Rmerge	0.045	0.028	0.306
Total number of observations	89114
Number of reflections	20985
<I/σ(I)>	16.8		3.1
Completeness [%]	98.8	100	91.2
Redundancy	4.2	4.3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	15-20% PEG, 0.1 M Tris-Cl, 0.01 M NiCl2