5HT7
Crystal structure of a transition-metal-ion-binding betagamma-crystallin from Methanosaeta thermophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.732, 52.821, 51.718 |
| Unit cell angles | 90.00, 100.48, 90.00 |
Refinement procedure
| Resolution | 24.250 - 1.862 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.961 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 4.000 | 1.860 |
| Rmerge | 0.045 | 0.028 | 0.306 |
| Total number of observations | 89114 | ||
| Number of reflections | 20985 | ||
| <I/σ(I)> | 16.8 | 3.1 | |
| Completeness [%] | 98.8 | 100 | 91.2 |
| Redundancy | 4.2 | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 15-20% PEG, 0.1 M Tris-Cl, 0.01 M NiCl2 |
