5HSZ
Structure of the K. pneumonia SlmA protein bound to the C-terminal tail of the cytoskeletal cell division protein FtsZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 69.261, 69.261, 325.613 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 59.982 - 2.300 |
R-factor | 0.2207 |
Rwork | 0.216 |
R-free | 0.26740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.534 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 59.982 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.060 |
Number of reflections | 34960 |
<I/σ(I)> | 11.2 |
Completeness [%] | 90.0 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 600 mM Sodium phosphate, 1200 mM potassium phosphate, 0.1 M imidazole pH 8.0, 0.2 M NaCl |