5HSP
MamM CTD M250L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.27 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 37.832, 94.914, 53.685 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.460 - 1.790 |
R-factor | 0.2018 |
Rwork | 0.197 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w5x |
RMSD bond length | 0.012 |
RMSD bond angle | 1.494 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.460 |
High resolution limit [Å] | 1.790 |
Rmerge | 0.110 |
Number of reflections | 9313 |
<I/σ(I)> | 16.5 |
Completeness [%] | 98.7 |
Redundancy | 12.3 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M (NH4)2SO4, 0.1 M BIS-TRIS pH=5.0 and 25% PEG 3350 |